| Uniprot identifier | Gene locus | Gene name | Soluble localization | Membrane localization | Predicted transmembrane helices | Predicted membrane topology | SignalP 6.0 Prediction | Predicted beta-barrel outer membrane protein | Gene product | PCC 6803 homolog | Average lumen over cytoplasm enrichment | Average periplasm over cytoplasm enrichment | Average lumen over periplasm enrichment |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Uniprot identifier | Gene locus | Gene name | Soluble localization | Membrane localization | Predicted transmembrane helices | Predicted membrane topology | SignalP 6.0 Prediction | Predicted beta-barrel outer membrane protein | Gene product | PCC 6803 homolog | Average lumen over cytoplasm enrichment | Average periplasm over cytoplasm enrichment | Average lumen over periplasm enrichment |
Column Descriptors #
Uniprot identifier: The identifier of the protein in Uniprot
Gene locus: The locus of the gene in PCC 7002
Gene name: The name of the gene in literature
Soluble Localization: The final localization of proteins after analysis 1 (thylakoid lumen vs cytoplasm samples to determine thylakoid lumen or cytoplasm localization) and analysis 2 (periplasm vs cytoplasm samples to determine periplasm/outer membrane or cytoplasm localization) in Spatial Proteomics Reveals Signal Sequence Characteristics Correlated with Localization in Cyanobacteria.
Membrane Localization: The final localization of membrane protiens (at least 1 predicted transmembrane helix) after comparing thylakoid lumen and periplasm samples in Spatial Proteomics Reveals Signal Sequence Characteristics Correlated with Localization in Cyanobacteria.
Predicted transmembrane helices: The number of predicted transmembrane helices by TMHMM Server v. 2.0
Predicted topology: The topology of the protein predicted by TMHMM Server v. 2.0.
SignalP 6.0 Prediction: The signal sequence prediction of the protein by SignalP-6.0 with the “Other” organism option (LIPO = Sec/SPII, NO_SP = no signal sequence predicted, PILIN = Sec/SPIII, SP = Sec/SPI, TAT = Tat/SPI, TATLIPO = TAT/SPII)
Predicted beta-barrel outer membrane protein: Whether a protein is predicted to have a beta-barrel fold characteristic of outer membrane proteins predicted by PRED-TMBB2, N = no beta-barrel prediction and Y = beta-barrel prediction
Gene product: The protein product of the gene
PCC 6803 homolog: The gene locus of the best reciprocal blast homolog of the PCC 7002 protein in PCC 6803
Average lumen over cytoplasm enrichment: The average enrichment of the protein in PsbQ or A2695 compared to GFP or CpcB samples in Spatial Proteomics Reveals Signal Sequence Characteristics Correlated with Localization in Cyanobacteria. The average enrichment of each replicate compared to each replicate (log2((PsbQ or A2695 replicate normalized TMT value +1)/(GFP or CpcB replicate normalized TMT value +1)).
Average periplasm over cytoplasm enrichment: The average enrichment of the protein in A1097 or A1761 compared to GFP or CpcB samples in Spatial Proteomics Reveals Signal Sequence Characteristics Correlated with Localization in Cyanobacteria. The average enrichment of each replicate compared to each replicate (log2((A1097 or A1761 replicate normalized TMT value +1)/(GFP or CpcB replicate normalized TMT value +1))
Average lumen over periplasm enrichment: The average enrichment of the protein in PsbQ or A2695 compared to A1097 or A1761 samples in Spatial Proteomics Reveals Signal Sequence Characteristics Correlated with Localization in Cyanobacteria. The average enrichment of each replicate compared to each replicate (log2((PsbQ or A2695 replicate normalized TMT value +1)/(A1097 or A1761 replicate normalized TMT value +1))